Nikon TE 2000 With PFS system 1

Nikon Timelapse SOP

 

We have 4 Nikon TE 2000 Timelapse microscope systems with PFS. The PFS  continuously corrects the focus at the plane of interest with its continuous optical offset feature. When the PFS is turned on the position of the glass bottom dish/plate is detected using a 777nm infra red light. The result is an end to out of focus drift. You should no longer have to adjust the focus knob after adding reagents or after stage movement. .The PFS is designed to work with glass bottom dishes. We have used plastic dishes but have had mixed results. If you are using plastic dishes or plates our advice is to switch off the PFS and use the system in the conventional way. The system is equipped with a Sutter lambda 10-3 controller, ASI MS2 XY stage with linear encoders, Photometrics CoolSnap HQ CCD camera and Metamorph 7.5 software.

 

 
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A: Transformed FVB cells                                                                                           B: Normal 208f cells

Timelapse images of normal and transformed cells moving in 2D. Below are computer generated graphs representing this movement in 40 cells of each type. Cell tracking can be done using ImageJ.

 

                                                                                        

This information about the TE2000 and more information can be found on the Nikon Website.

 


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Last modified: 02/25/16