The above diagram represents the optical path on a
laser point scanning confocal microscope.A small diaphragm, situated in a conjugated focal plane,
prevents out of focus light reaching the detected. The diameter of this
diaphragm controls the depth of the optical section.Images
captured will have improved Z resolution and fine detail that is
normally obscured by the out of focus blur assosiated with wide-field
fluorescent microscopy will be seen.
Z sectioning allows the optically collection of images through the
depth of the sample. This series of images can then be reconstructed into a
high resolution 3D image.
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Mitotic cells in 3D and Gallery showing optical Z stack. L.Black R11