LIFA FLIM-TIRF Live Cell Imaging system

LIFA FLIM-TIRF SOP

LIFA_FLIM_WF

This system has a LIFA -Lambert Instruments Fluorescence Attachment for lifetime imaging. It is attached to a Nikon TE2000 microscope. The LIFA system calculates lifetimes based on the frequency domain method  by using a modulated light source and a modulated image intensifier as detector. This Lambert system is coupled through our custom TIRF condenser to make it a FLIM-TIRF system. It has two Omicron lasers attached (473nm and 561nm). The system can be used as a widefield system with  LED light sources (407, 445, 453, 467, 469, 491 and 535nm are available)

 

 


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Copyright 2009 Beatson Advanced Imaging Reaource
Last modified: 02/25/16