Photoactivation.

Photoactivation is increasingly being used to  investigate intracellular protein movement. Here the fluorophore only becomes active and fluorescent after being excited with a laser that causes a structural change to the molecule.

The above pictures are taken from a photoactivation movie. They show a line of activation through the lamellipodia of a cell. The GFP_F diffuses quickly after activation with a 405nm laser. Images taken on the Olympus FV1000.

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Human Fibroblasts and mitochondrial dynamics looking at levels of fission and fusion in the cells. Mito-dsred with mitochondrial localisation sequence and Photoactivatable GFP. Images captured using the NIkon A1R confocal microscope.

 


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Last modified: 02/25/16