Photoactivation is increasingly being used to investigate intracellular
protein movement. Here the fluorophore only becomes active and fluorescent after
being excited with a laser that causes a structural change to the molecule.
The above pictures are taken from a photoactivation movie. They show a line
of activation through the lamellipodia of a cell. The GFP_F diffuses quickly
after activation with a 405nm laser. Images taken on the Olympus FV1000.
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Human Fibroblasts and mitochondrial dynamics looking at levels of fission and
fusion in the cells. Mito-dsred with mitochondrial localisation sequence and
Photoactivatable GFP. Images captured using the NIkon A1R confocal microscope.