Fiji is an image processing package based on image J. Fiji stands for Fiji is
Just ImageJ. It is free to download from the developers website and has some
detailed tutorials on how use some of its plugins.
It has some advantages over image J as you can contact some of the developers
for assistance and help.
Colocalization Analysis Tutorial
When using this tutorial the test image must be downloaded and then opened in
Image J can display, edit, analyze, process, save and print 8-bit, 16-bit and
32-bit images. It can read many image formats including TIFF, PNG, GIF, JPEG,
BMP, DICOM, FITS, as well as raw formats.
Image J supports image stacks, a series of images that share a single window,
and it is multithreaded, so time-consuming operations such as image file reading
can be performed in parallel with other operations.
Image J can calculate area and pixel value statistics of user-defined selections
and intensity threshold objects. It can measure distances and angles.( We use
this software to Track timelapse cells)
It can create density histograms and line profile plots. It supports standard
image processing functions such as logical and arithmetical operations between
images, contrast manipulation, convolution, Fourier analysis, sharpening,
edge detection and median filtering. It does geometric transformations such as
scaling, rotation and flips. The program supports any number of images
simultaneously, limited only by available memory.
Image J was designed with an open architecture that provides extensibility via
Java plugins and recordable macros. Custom acquisition, analysis and processing
plugins can be developed using Image J's built-in editor and a Java compiler.
User-written plugins make it possible to solve almost any image processing or
Image J Wright Cell Imaging Facility is the version of Image J used at the
time to open from the NAL.
If you are going to be using this software a lot I would suggest you download it
to the hard drive of your PC. The BAIR Facility has copies of Image J on USB's
that can be borrowed.
to image J manual
How to measure intensities of images using Image J
Tracking cells using Image J