FiJI ImageJ.

Fiji is an image processing package based on image J. Fiji stands for Fiji is Just ImageJ. It is free to download from the developers website and has some detailed tutorials on how use some of its plugins.
It has some advantages over image J as you can contact some of the developers for assistance and help.

Fiji download

Scalebar ImageJ

Colocalization Analysis Tutorial

When using this tutorial the test image must be downloaded and then opened in fiji

Colocalization presentation

Image J

Image J can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, PNG, GIF, JPEG, BMP, DICOM, FITS, as well as raw formats.
Image J supports image stacks, a series of images that share a single window, and it is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations.
Image J can calculate area and pixel value statistics of user-defined selections and intensity threshold objects. It can measure distances and angles.( We use this software to Track timelapse cells)
It can create density histograms and line profile plots. It supports standard image processing functions such as logical and arithmetical operations between images, contrast manipulation, convolution, Fourier analysis, sharpening, smoothing,
edge detection and median filtering. It does geometric transformations such as scaling, rotation and flips. The program supports any number of images simultaneously, limited only by available memory.
Image J was designed with an open architecture that provides extensibility via Java plugins and recordable macros. Custom acquisition, analysis and processing plugins can be developed using Image J's built-in editor and a Java compiler.
User-written plugins make it possible to solve almost any image processing or analysis problem.

Image J(WCIF)

Image J Wright Cell Imaging Facility is the version of Image J used at the Beatson. It is accessed via the NAL. Because it uses Javascript it can take some time to open from the NAL.
If you are going to be using this software a lot I would suggest you download it to the hard drive of your PC. The BAIR Facility has copies of Image J on USB's that can be borrowed.

Link to image J manual

How to measure intensities of images using Image J

Tracking cells using Image J



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Copyright 2009 Beatson Advanced Imaging Reaource
Last modified: 02/25/16