A2780 GFP_RCP mCherry src                              Colocalized Pixels                                                Scatter Plot Pearson's Correlation Coefficient(r)

Images from R20

Confocal microscopes optical sectioning capability make them the ideal tool for capturing images for colocalization studies. Colocalization can be used to measure the percentage overlap between two proteins that have been labelled with different fluorescent dyes or proteins. Several colocalization software packages are available and it can be difficult known which one to choose and which calculation method to use. We favour using Image J or FIJI for our colocalization studies.

There are two basic methods for colocalization analysis which can be found on the FIJI site.

1.Pixel intensity Spatial Correlation Analysis

2.Object Based Overlap Analysis

Papers to read before you start:




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Copyright 2009 Beatson Advanced Imaging Reaource
Last modified: 02/25/16