mCherryLC3/lamp2Alexa488/DAPI JO'Prey R5.
Nikon AIR SOP
The Nikon A1 system is
a fully automated,
highly sensitive confocal microscope system. It has the capability to scan very
rapidly (230 frames per second in a 512 x 64 pixel image and 30 fps at 512 x 512). This
is due to the A1R's
scanning systems. Firstly there is a conventional sawtooth scanning system – galvano, (This is used for high resolution imaging where speed is not essential). Secondly
also a fast resonant scanner which is used for applications where the
need for speed outweighs the need for high resolution.
The two scanning systems can be operated in rapid succession or simultaneously. This makes
it possible to stimulate the specimen during observation. As a result, the rapid
protein dynamics that occur immediately after the laser has stimulated the cell can be accurately captured and analysed. The
A1R also has a
spectral detector, based on a conventional 32 cathode PMT. The system is built around
a Nikon Ti Eclipse microscope with PFS. This makes it the perfect confocal to use
for live cell timelapse imaging
Scan head with regular galvanometer mirrors and
4 laser system with AOTF 405LD 38mw,561nmDPSS
25mW,638nmLD 10mW and
multi-line argon gas laser (457nm, 488nm, 514nm).
XY,XYT,XYZ,XYZT and multipoint scanning .Galvano
pixel max 4096x4096 pixels. Resonant pixel max 512x512 pixels.
Simultaneous photoactivation/bleaching and
4 channel PMT detector and 32channel PMT
Transmitted light detector
3d and 4D imaging.
This information and additional information
about the A1R can be found on the Nikon website: