Nikon TIRF System
Nikon TIRF SOP
TIRF multi spec alignment
This system is appropriate for live cell fluorescent protein imaging. The
system is built around a Nikon Eclipse inverted stand with
custom optics for illumination of GFP and RFP at 473nm and 561nm, respectively.
Red and green fluorescent proteins are imaged simultaneously onto a high
sensitivity Photometrics Evolve 512 camera using a DualView DV2 emission splitter.
The system is also equipped with a 405nm laser for photo-activation and FRAP.