Nikon TIRF System

Nikon TIRF SOP

TIRF multi spec alignment

This system is appropriate for live cell fluorescent protein imaging. The system  is built around a Nikon Eclipse inverted stand with custom optics for illumination of GFP and RFP at 473nm and 561nm, respectively. Red and green fluorescent proteins are imaged simultaneously onto a high sensitivity Photometrics Evolve 512 camera using a DualView DV2 emission splitter. The system is also equipped with a 405nm laser for photo-activation and FRAP.

 

 


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Copyright 2009 Beatson Advanced Imaging Reaource
Last modified: 02/25/16