Nikon FLIM-TIRF SOP

This system has a LIFA -Lambert Instruments Fluorescence Attachment for lifetime imaging. It is attached to a Nikon Ti microscope with PFS. The LIFA system calculates lifetimes bases on  the frequency domain method  by using a modulated light source and a modulated image intensifier as detector. This Lambert system is coupled through our custom TIRF condenser to make it a FLIM-TIRF system. It has two Omicron lasers attached ( 473nm and 561nm).

The system can also be used for widefield FLIM using LED light sources which are also fitted to the system.(407,445,453,467,469,491 and 535nm are available)

 


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Copyright 2009 Beatson Advanced Imaging Reaource
Last modified: 02/25/16