Nikon FLIM-TIRF SOP
This system has a LIFA -Lambert Instruments
Fluorescence Attachment for lifetime imaging. It is attached to a Nikon Ti
microscope with PFS. The LIFA system calculates lifetimes bases on the frequency domain
method by using a modulated light source and a modulated image intensifier as detector.
This Lambert system is coupled through our custom TIRF condenser to make it a
FLIM-TIRF system. It has two Omicron lasers attached ( 473nm and 561nm).
The system can also be used for widefield FLIM using LED light sources which
are also fitted to the system.(407,445,453,467,469,491
and 535nm are available)