TRIMScope.                                                                                   Invasive cancer cells on the surface of an organotypic sample

 

The LaVision TRIM microscope is a high-resolution multiphoton system able to image small groups of cells down to the level of subcellular structure and generate three-dimensional reconstructions. This system is used to image neurons of the central nervous system and epithelial cells within the crypts of the colon. The Lavision TRIM scan head is mounted on a Nikon Eclipse inverted microscope. Illumination is provided by a Chameleon ll Ultra fast laser (680nm-1080nm 3.3W peak) which also pumps an APE optical parametric oscillator (1050nm-1320nm, 500mW peak) to provide excitation of red fluorescent proteins.

Applications:

•Offers fluorescent and second harmonic generated imaging of samples from cell culture through to whole tissue samples via intermediate systems such as organotypic invasion assays (artificial skin)
•Example samples:
•cells on glass / CDM / collagen invasion assays;
•organotypic samples which are a mix of collagen type 1 and fibroblasts, providing a model of stroma with both fluorescent cultured cells and fluorescent fibroblasts possible. This allows the 3-d visulisation of the interaction between tumour cells and the host stromal fibroblasts. The nature of this assay easily allows the direct genetic and or drug modification of cellular behavior to be studied over a course of approximately a month
•Sample slices – the quantity of collagen can be quantified in various locations
•Tissue samples from genetic models, transplant generated models
•Techniques available:
•Intensity fluorescence and second harmonic generation
•Full range of fluorophores accessible to imaging from DAPI through to far-reds
•Fluorescence lifetime imaging (FLIM) across all fluorophores and autofluorescence e.g. NAD(P)H
•Z-stack, tile scanning, time course

 

 

This information and more information about the system can be found on the LaVision website.

 

 


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Copyright © 2009 Beatson Advanced Imaging Reaource
Last modified: 02/25/16